if possible the human bacterial commensal qualified for genetic modification are: Propionibacterium propionicum, Corynebacterium amycolatum, Actinomyces massiliensis, Bacteroides thetaiotaomicron. all the more if possible the human bacterial commensal targeted for genetic modification is Propionibacterium propionicum.
262、细菌噬菌体可选自长尾噬菌体科(非限制性地比如以下属：cam病毒、lika病毒、r4病毒、acadian病毒、cooper病毒、pg1病毒、pipefish病毒、rosebush病毒、brujita病毒、che9c病毒、hawkeye病毒、plot病毒、jersey病毒、k1g病毒、sp31病毒、lmd1病毒、una4病毒、bongo病毒、rey病毒、butters病毒、charlie病毒、redi病毒、baxter病毒、nymphadora病毒、bignuz病毒、fishburne病毒、phayonce病毒、kp36病毒、rogue1病毒、rtp病毒、t1病毒、tls病毒、ab18病毒、amigo病毒、anatole病毒、andromeda病毒、attis病毒、barnyard病毒、bernal13病毒、biseptima病毒、bron病毒、c2病毒、c5病毒、cba181病毒、cbast病毒、ceci病毒、che8病毒、chi病毒、cjw1病毒、corndog病毒、cronus病毒、d3112病毒、d3病毒、decurro病毒、demosthenes病毒、doucette病毒、e125病毒、eiau病毒、ff47病毒、gaia病毒、giles病毒、gordon病毒、gordtnk病毒、harrison病毒、hk578病毒、hk97病毒、jenst病毒、jwx病毒、kellezio病毒、korra病毒、l5病毒、lambda病毒、laroye病毒、liefie病毒、marvin病毒、mudcat病毒、n15病毒、nonag病毒、np1病毒、omega病毒、p12002病毒、p12024病毒、p23病毒、p70病毒、pa6病毒、pamx74病毒、patience病毒、pbi1病毒、pepy6病毒、pfr1病毒、phic31病毒、phicbk病毒、phieta病毒、phifel病毒、phijl1病毒、pis4a病毒、psa病毒、psimuna病毒、rdjl病毒、rer2病毒、sap6病毒、send513病毒、septima3病毒、seurat病毒、sextaec病毒、sfi11病毒、sfi21dt1病毒、sitara病毒、sk1病毒、slash病毒、smoothie病毒、soups病毒、spbeta病毒、ssp2病毒、t5病毒、tank病毒、tin2病毒、titan病毒、tm4病毒、tp21病毒、tp84病毒、tria病毒、trigintaduo病毒、vegas病毒、vendetta病毒、wbeta病毒、wildcat病毒、wizard病毒、woes病毒、xp10病毒、ydn12病毒和yua病毒)。
The sequence encoding Cms1 (the whole protein or a fragment thereof) as Employed in the context of the invention is disclosed in Intercontinental patent software WO2017/141173.
In a particular embodiment, the donor bacterial cell with the invention comprises the above-defined helper 免費體驗金 phage.
The current inventors in fact made herein a particular conditional origin of replication derived from PICIs.
The bacterial shipping and delivery vehicles are typically well prepared from bacterial virus. The bacterial shipping automobiles are typically picked out if you want in order to introduce the vector into your targeted bacteria.
to resolve this difficulty, the existing inventors have herein made a different system that ensures that DNA payloads the moment sent in concentrate on bacteria can't replicate in the concentrate on bacteria but still Specific the gene(s) of curiosity in a amount that is sufficient to exert the envisioned result to the microbes or to the host, without the want of an antibiotic resistance variety marker within the DNA payload, and with no will need of a variety phase by having an antibiotic.
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In an alternate embodiment, when reported vector is comprised inside a bacterial shipping and delivery automobile, explained auto doesn't bind to explained probiotic and mentioned probiotic is not really affected by mentioned vector.
whereby, when shipped into stated qualified receiver bacterial mobile, mentioned nucleic acid of fascination makes claimed offered effect on said specific receiver bacterial mobile whilst explained vector is not really replicated in explained focused receiver bacterial cell.
In a specific embodiment, when reported origin of replication is derived from phage-inducible chromosomal islands (PICIs), said conditional origin of replication is Energetic in reported donor bacterial cell due to the fact stated donor bacterial cell expresses a rep protein, especially a primase-helicase, specifically a primase-helicase of sequence SEQ ID NO: 8.
As made use of herein, the phrase “conjugative plasmid” refers to the plasmid that is certainly transferred from a person bacterial mobile to another for the duration of conjugation and also a “donor bacterium”, as made use of herein, is then a bacterium that may be capable of transferring a conjugative plasmid to a different bacterium.
In a selected embodiment, claimed donor bacterial mobile is often a output cell line, specifically a mobile line generating packaged phagemids such as the vector of the creation.
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